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Activation of NLRP3 inflammasome
Activation of NLRP3 inflammasome

Exploratory Research

Measured value Ratio to control
Animal species Others
Indication of the result The ratio of the value of NLRP3 inflammasome activity to that for the control condition with DMSO is shown. Values greater than 1 indicate activation of NLRP3 inflammasome.
Target diseases of assay Immune system, Allergy
Assay level Cell-based
Protocol of assay 1) J774 cells were loaded with membrane potential-sensitive fluorescent dye DiBAC4(3) and seeded on 96-well plates. 1) 0.2 µl of test drug solution and 199.8 μl of assay buffer were added to each well, and the cells were incubated for 90 min at 37 °C. 3) NLRP3 inflammasome activity was measured by the fluorescence intensity using an excitation wavelength of 493 nm and an emission wavelength of 516 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
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