|Measured value||Ratio to control|
|Indication of the result||The ratio of the value of NLRP3 inflammasome activity to that for the control condition with DMSO is shown. Values greater than 1 indicate activation of NLRP3 inflammasome.|
|Target diseases of assay||Immune system, Allergy|
|Protocol of assay||1) 0.2µl of test drug is mixed with 199.8 μl of assay buffer,
2) The mixture is incubated for 90 min at 37°C.
3) NLRP3 inflammasome activity is measured by the fluorescence using an excitation wavelength of 493 nm and an emission wavelength of 516 nm.