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Anti-influenza viral activity
Anti-influenza viral activity

Exploratory Research

Measured value Others
Animal species Others
Indication of the result The relative cell densities (%) against average cell densities of untreated control wells without infection (100 %) and untreated control wells with infection (0 %) were shown. Larger values indicate stronger anti-viral activity.
Target diseases of assay Infective disease
Assay level Cell-based
Protocol of assay 1) MDCK cells were seeded onto 96-well plates. 2) Next day, MDCK cells were infected with influenza virus, and Kampo extracts, medicinal plant extracts (100 μg/mL), or compounds (10 μM) were added. The culture plates were incubated for 2 days. 3) Cells were fixed with 70 % EtOH and stained with 0.5 % crystal violet. 4) After washing with tap water and air drying, cell density was measured by the absorbance at 560 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
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