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Antitrypanosomal activity
Antitrypanosomal activity

Exploratory Research

Measured value Others
Animal species Others
Indication of the result Minimum lethal concentration to kill Trypanosoma cruzi is shown. 999 mean that no lethal effect was observed even at the maximum concentration in the experiment.
Target diseases of assay Infective disease
Assay level Cell-free
Protocol of assay 1) Crude drug extracts and compounds were dissolved in water and DMSO, respectively. 2) 185 μl of PBS, 10 μl of T. cruzi epimastigotes, and 5 μl of test drug solution were mixed in a 96-well plate, and the mixture was incubated for 24 h at 27 °C. 3) T. cruzi was observed under microscope, and the minimum lethal concentration (MLC) was determined.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
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