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Cytotoxicity against HL60 cells
Cytotoxicity against HL60 cells

Exploratory Research

Measured value Ratio to control (%)
Animal species Human
Indication of the result Dehydrogenase activity detection by WST-8 in viable HL60 cells with 0.1% DMSO or water is determined as 100%. Data of the drug treated wells are shown as growth rate compared to positive contorol.
Target diseases of assay Cancer
Assay level Cell-based
Protocol of assay 1) HL60 cells were plated at 10000 cells/well on 96-well plates with 100 μL of a culture medium. 2) After treatment with the compounds for 72 h, 10 μL of the WST-8 reagent (Dojindo, Kumamoto, Japan) was added into each well followed by incubation for 3 h at 37 °C. 3) Absorbance was then measured at 450 nm using a SpectraMax PLUS 384 microplate reader (Molecular Devices, Sunnyvale, CA, USA).
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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