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Cytotoxicity against HepAD38 cells
Cytotoxicity against HepAD38 cells

Exploratory Research

Measured value Ratio to control (%)
Animal species Human
Indication of the result The ratio (%) of viable cell number under test drug treatment to that for the control condition (PBS) is shown. Values less than 100 indicate cytotoxic effect.
Target diseases of assay Infective disease
Assay level Cell-based
Protocol of assay 1) HepAD38.7 cells were seeded on 96 wells plate in tetracycline-positive DMEM medium (100 μl each well). 2) Next day, medium was replaced with DMEM without tetracycline and 1 μl of test drugs (final concentration 100 μg/ml) were added. 3) The culture plates were incubated in the same conditions described above for 9 days. The medium and test drugs were replaced every 3 days. 4) The cultured plates were observed for cell viability with the CellTiter-Glo reagents.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
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