Menu  → 【Exploratory Research】Methods of bioassay  → 
Cytotoxicity against Jurkat cell
Cytotoxicity against Jurkat cell

Exploratory Research

Measured value Inhibition (%)
Animal species Human
Indication of the result The rate of decrease (%) in viable cell number against the control condition without test drug treatment is shown. Positive values indicate cytotoxic effect.
Target diseases of assay Cancer
Assay level Cell-based
Protocol of assay 1) Jurkat cells were incubated with or without test drug for 24 h at a concentration of 5x10^4 cells/well. 2) WST8 reagent was added to each well, and the cells were further incubated for 3 h. 3) Cell viability was quantified by measuring light absorbance at 490 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

TOPICS

Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

Traditional Medical & Pharmaceutical Database


Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
TEL: +81-76-434-7601
FAX: +81-76-434-5064