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Inhibition of NFkB reporter assay
Inhibition of NFkB reporter assay

Exploratory Research

Measured value Inhibition (%)
Animal species Mouse
Indication of the result The inhibitory activity (%) of each extracts or compounds in luciferase reporter assay was shown. The luciferase activity of control group (medium for extracts or DMSO for compounds) should be 100%.
Target diseases of assay Cancer
Assay level Cell-based
Protocol of assay "1) Seed 4T1-NFkB-Luc2 cells and incubate for 3hrs (37℃)
2) Add extracts or compounds, then incubete for 24hrs
3) Add luciferin and read luminescence after 30min "
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Information on genetic analysis

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