|Indication of the result||Evaluation of presence or absence of the effects of drug treatment on the activation of BMP4-Smad1-Sox9 signaling pathway in activated mouse mesangial cells are shown.|
|Target diseases of assay||Others|
|Protocol of assay||1) Plate mouse mesangial cells onto 10-cm plate and grow to 50% confluent.
2) Add 50 μl of drug solution to the plates in duplicate.
3) Observe the status of the cells under microscope.
4) Extract protein from the treated cells after 24h-stimulation.
5) Analyze the protein levels of βactin, Smad1, pSmad1, and Sox9 by Western blot method.