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Inhibitory effect on Btg2 gene expression
Inhibitory effect on Btg2 gene expression

Exploratory Research

Measured value Ratio to control (%)
Animal species Mouse
Indication of the result The ratio (%) of Btg2 gene expression under test drug treatment to that for the control condition without test drug is shown. Values less than 100 indicate inhibition of Btg2 gene expression.
Target diseases of assay Brain, Nervous system
Assay level Cell-based
Protocol of assay 1) Neuro2a cells were treated with test drug except for the control condition. 2) 100 µM dexamethasone (DEX) was added, and the cells were incubated for 24 h. 3) RNA was taken from the cells, and real-time PCR was performed using an ABI PRISM 7900HT Sequence Detection System with the THUNDERBIRD qPCR Mix. 4) The value of Btg2 gene expression was normalized using the value of Gapdh gene expression.
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)



Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
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