Menu  → 【Exploratory Research】Methods of bioassay  → 
Inhibitory effect on MTH1 enzyme activity
Inhibitory effect on MTH1 enzyme activity

Exploratory Research

Measured value Inhibition (%)
Animal species Others
Indication of the result The rate of decrease (%) of MTH1 enzyme activity against the control condition without test drug treatment is shown. Positive values indicate inhibitory effect on MTH1 enzyme activity. Negative values are replaced with 0.
Target diseases of assay Cancer
Assay level Cell-free
Protocol of assay 1) 1 μl of test drug, 44 μl of assay buffer, and 4 μl of diluted MTH1 were mixed in 96-well plate. The mixture was incubated for 15 min at 22 °C. 2) 100 μl of malachite green solution was added to wells. The mixture was incubated for 20 min at 22 °C. 3) MTH1 enzyme activity was measured by the absorbance at 620 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

TOPICS

Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

Traditional Medical & Pharmaceutical Database


Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
TEL: +81-76-434-7601
FAX: +81-76-434-5064