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Inhibitory effect on NFAT promoter activity
Inhibitory effect on NFAT promoter activity

Exploratory Research

Measured value Ratio to control (%)
Animal species Human
Indication of the result The ratio (%) of the value of NFAT promoter activity under test drug treatment to that for the control condition (vehicle only) is shown. Values less than 100 indicate inhibition of NFAT promoter activity.
Target diseases of assay Immune system, Allergy
Assay level Cell-based
Protocol of assay 1) Jurkat cells were transfected with pNFAT-luciferase reporter gene. The cells were incubated with test drugs for 30 min at 37 ˚C in CO2 incubator. 2) The cells were stimulated with PMA and ionomycin and incubated for further 7 h. 3) NFAT promoter activity was measured by the luciferase activity of the cell extracts using Picagene Luminescence Kit.
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)



Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
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