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Inhibitory effect on NO production
Inhibitory effect on NO production

Exploratory Research

Measured value Ratio to control (%)
Animal species Mouse
Indication of the result The ratio (%) of the amount of NO production under test drug treatment to that for the control condition without test drug is shown. Values less than 100 indicate inhibition of NO production.
Target diseases of assay Brain, Nervous system
Assay level Cell-based
Protocol of assay 1) BV-2 cells were seeded at a density of 7.5×10^4 cells/well in 96-well plates for Griess assay. Test drug solution was added to the wells, and the cells were incubated for 30 min. 2) LPS (0.1 μg/mL) was added to the medium, and the cells were incubated for 24 h. 3) Cell-free supernatants were collected and mixed with an equal volume of Griess reagent (1 % sulfanilamide, 0.1 % naphthylethylenediamine dihydrochloride, and 2.5 % phosphoric acid). 4) Absorbance was measured at 570 nm with a microplate reader (Bio-Rad, Hercules, CA, USA) and nitrite concentrations were determined with reference to a calibration curve prepared with sodium nitrite standards.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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