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Inhibitory effect on NS2B-NS3 enzyme activity
Inhibitory effect on NS2B-NS3 enzyme activity

Exploratory Research

Measured value Ratio to control (%)
Animal species Others
Indication of the result The ratio (%) of NS2B-NS3 enzyme activity against the negative control (100 %) and the positive control (0 %) is shown. Values less than 100 indicate the inhibitory effect on NS2B-NS3 enzyme activity.
Target diseases of assay Infective disease
Assay level Cell-free
Protocol of assay 1) 3 mL of the test substance solution (3 mg/mL or 3 mM) and 27 μL of buffer were mixed in a 384-well plate. 2) 10 mL of substrate (BOC-GRR-MCA) and 17 mL of enzyme (NS2B gly NS3) were added. The mixture was left at room temperature for 20 min. 3) The NS2B-NS3 enzyme activity was measured by fluorescence intensity (excitation wavelength 360 nm, fluorescence wavelength 440 nm).
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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