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Inhibitory effect on PAI-1 production
Inhibitory effect on PAI-1 production

Exploratory Research

Measured value Ratio to control
Animal species Human
Indication of the result The ratio of PAI-1 concentration under test drug treatment to that for the control condition with DMSO is shown. Values less than 1 indicate inhibition of PAI-1 production.
Target diseases of assay Cardiovascular system
Assay level Cell-based
Protocol of assay 1) EA.hy926 cells were cultured for 48 h in gelatin-coated 96-well plates. 2) The medium was changed to DMEM (D-Glucose 1g/L) containing 1 % FBS, and the cells were cultured for 24 h. 3) The medium was changed to DMEM containing 1 % FBS and test drug solution or DMSO, and the cells were incubated for 3 h. 4) The medium was changed to DMEM containing TNF-α (10 ng/mL), and the cells were incubated for 24 h. 5) The medium was centrifuged (1000 rpm, 5 min, 4 °C), and the PAI-1 concentration in the supernatants was measured using a total human PAI-1 ELISA.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
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