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Inhibitory effect on the enzyme activity of HDAC
Inhibitory effect on the enzyme activity of HDAC

Exploratory Research

Measured value Ratio to control (%)
Animal species Human
Indication of the result The ratio (%) of the value of histone deacetylase (HDAC) enzyme activity to that for the control condition with 0.1 % DMSO or water is shown. Values less than 100 indicate inhibition of HDAC enzyme activity.
Target diseases of assay Cancer
Assay level Cell-free
Protocol of assay An HDAC Activity Assay Kit (ab156064, Abcam) was used. 1) Assay buffer, substrate (fluorescence-labeled acetylated peptide) solution, and test drug solution or its vehicle only (control) were mixed. 2) HDAC enzyme was added to each well, and the mixtures were incubated for 20 min at room temperature. 3) Stop solution was added. 4) Developer was added in order to cleave the deacetylated substrates produced by the HDAC enzyme reaction, and the mixtures were incubated for 10-40 min at room temperature. 5) The HDAC enzyme activity was measured by the fluorescence intensity (excitation wavelength 355 nm, emission wavelength 460 nm).
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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