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Inhibitory effect on the enzyme activity of HDAC
Inhibitory effect on the enzyme activity of HDAC

Exploratory Research

Measured value Ratio to control (%)
Animal species Human
Indication of the result The ratio (%) of the value of Histone Deacetylase (HDAC) enzyme activity to that for the control condition with 0.1 % DMSO or water is shown. Values less than 100 indicate inhibition of HDAC enzyme activity.
Target diseases of assay Others
Assay level Cell-free
Protocol of assay An HDAC activity assay [ab156064] Abcam (Cambridge, MA, USA) was used. The reaction is initiated and fluorescence intensity is measured by mixing simultaneously fluorescence-labeled acetylated peptide (which acts as substrate), HDAC (enzyme) and the developer. The reaction is not stopped, so it is necessary to measure fluorescence intensity at regular intervals after the reaction is initiated. The steps are as follows, 1)Prepare samples
2) Prepare reaction wells (HDAC Assay Buffer and substrate)
3) Add HDAC enzyme (control HDAC or your sample) to each well Incubate for 20 min at room temperature
4) Add Stop Solution & Developer to each well
5) Incubate for 10-40 min at room temperature.
6) Measure Fluorescence Intensity at Ex/ Em = 355/ 460 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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