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Inhibitory effect on the enzyme activity of SuperScript II reverse transcriptase
Inhibitory effect on the enzyme activity of SuperScript II reverse transcriptase

Exploratory Research

Measured value Ratio to control (%)
Animal species Others
Indication of the result The ratio (%) of the enzyme activity of SuperScript II reverse transcriptase under test drug treatment to that for the control condition with water is shown. Values less than 100 indicate the inhibition of the enzyme activity.
Target diseases of assay Others
Assay level Cell-free
Protocol of assay 1) 3 μl of Test drug was mixed with 20 μl of assay buffer including 40 U of SuperScript II reverseh transcriptase. The mixture was incubated for 2 h at 37 °C. 2) 20 μl of ABTS substrate solution was added to wells. The mixture was heated to 37 °C. 3) The enzyme activity of SuperScript II reverse transcriptase was measured by the absorbance at 405 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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