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Inhibitory effect on the enzyme activity of allosidase
Inhibitory effect on the enzyme activity of allosidase

Exploratory Research

Measured value Inhibition (%)
Animal species Human
Indication of the result The rate of decrease (%) in the enzyme activity of allosidase against the control condition is shown. Positive values indicate inhibition of the enzyme activity. Negative values were replaced with 0.
Target diseases of assay Infective disease
Assay level Cell-based
Protocol of assay 1) HeLa cells were cultured in a 96-well plate. 2) The medium was replaced with fresh medium, and fluorescent substrates and DMSO solution of test drug were added. Cells were incubated. 3) The plates were washed by PBS three times. The enzyme activity was measured by the fluorescence intensity with a multi-mode microplate reader.
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)



Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

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