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Inhibitory effect on the enzyme activity of amylase
Inhibitory effect on the enzyme activity of amylase

Exploratory Research

Measured value Ratio to control (%)
Animal species Others
Indication of the result The ratio (%) of the enzyme activity of amylase under test drug treatment to that for the control condition with Milli-Q water is shown. Values less than 100 indicate inhibition of the enzyme activity.
Target diseases of assay Digestive system
Assay level Cell-free
Protocol of assay 1) MES buffer (1000 μl) was used as a reference, and the automatic zero-point adjustment was performed in the spectrophotometer. 2) MES buffer, α-amylase, and Milli-Q water or test drug solution were added in a cell, and the mixture was incubated at 37 °C for 10 min. 3) The substrate solution was added into the cell, and the reaction rate (mAbs/min) was estimated from the measurements of the absorbance at 405 nm for 2 min.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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