|Measured value||Ratio to control (%)|
|Indication of the result||Remaining releasing activities of 2-chloro-4-nitorphenol from Gal-Glc-CNP by a-amylase in the presence of test samples.|
|Target diseases of assay||Digestive system|
|Protocol of assay||1. MES buffer (1000 ml) was used as a reference.
2. Add MES buffer, a-amylase, and a sample in a cell, and incubate it at 37˚C for 10 min.
3. Add the substrate solution into the cell and monitor the change in absorvance at 405 nm.
4. Calculate the remaining activitiy for each sample.