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Inhibitory effect on the enzyme activity of serine-carboxyl protease
Inhibitory effect on the enzyme activity of serine-carboxyl protease

Exploratory Research

Measured value Inhibition (%)
Animal species Others
Indication of the result The rate of decrease (%) in the enzyme activity of serine-carboxyl protease under test drug treatment against the control condition without test drug is shown. Larger values indicate stronger inhibitory effect.
Target diseases of assay Brain, Nervous system
Assay level Cell-free
Protocol of assay 1) 5 μL of test drug solution was added to 15 μL of serine-carboxyl protease (1.2 μg of kumamolisin per mL in 50mM acetate buffer, pH 3.4) of each tube. These mixtures were incubated at 60 °C for 10 min. 2) 180 μL of substrate (4 μM、Nma-Lys-Pro-Ile-ProArg-Phe-Arg-Leu(Dnp)-NH2 in 50 mM acetate buffer, pH 3.4) was added to each tube and was incubated at 60 °C for 15 min. 3) The enzyme activity was measured by the fluorescence intensity (excitation 340 nm, emission 440 nm) using a TECAN infinite M200 microplate reader.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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