Menu  → 【Exploratory Research】Methods of bioassay  → 
Inhibitory effect on the enzyme activity of streptococcal NADase
Inhibitory effect on the enzyme activity of streptococcal NADase

Exploratory Research

Measured value Inhibition (%)
Animal species Others
Indication of the result The rate of decrease (%) in the enzyme activity of streptococcal NADase under test drug treatment against the control condition with water is shown. (Comment by a DB administrator: Positive values may indicate stimulatory effect on the NADase enzyme activity because the amount of the remaining substrate that were not hydrolyzed by NADase decreased as compared to the control condition.)
Target diseases of assay Infective disease
Assay level Cell-free
Protocol of assay 1) Test drug (10 μl solution) was mixed with 10 μl of diluted NADase and 80 μl of assay buffer (final concentration: 2 mM NAD+, 0.1 M Na-phosphate buffer, pH 7.4) in a 96-well plate. The mixture was incubated for 20-60 min at 23 °C. 2) 200 μl of stop solution of 0.1 M Na2SO3 was added to wells. The mixture was incubated for 30 min at 37 °C. 3) NADase activity was measured by the absorbance of NADH drived from remaining NAD+ using a measurment wavelength of 340 nm and a reference wavelength of 630 nm.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

TOPICS

Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

Traditional Medical & Pharmaceutical Database


Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
TEL: +81-76-434-7601
FAX: +81-76-434-5064