Menu  → 【Exploratory Research】Methods of bioassay  → 
MHC class I antigen presentation
MHC class I antigen presentation

Exploratory Research

Measured value Ratio to control (%)
Animal species Mouse
Indication of the result The value of IL-2 concentration with water is determined as 100%. Data of drug treatment are revealed as increasing rate (%) against the control.
Target diseases of assay Immune system, Allergy
Assay level Cell-based
Protocol of assay 1. DC2.4 cells suspended in AIM-V onto 96-well plates.
2. Incubation for 30min.
3. Cultivation for 1 hr after addition of test agents to the plates
4. Cultivation for 20 hr after addition of Lipofectine and OVA solution.
5. Fixation with 0.05 % glutaraldehyde
6. CD8-OVA1.3 suspended in AIM-V onto 96-well plates
7. Measurement of IL-2 concentration by ELISA after 20 hr.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

TOPICS

Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

Traditional Medical & Pharmaceutical Database


Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
TEL: +81-76-434-7601
FAX: +81-76-434-5064