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MHC class II antigen presentation
MHC class II antigen presentation

Exploratory Research

Measured value Ratio to control (%)
Animal species Mouse
Indication of the result The value of IL-2 concentration with water is determined as 100%. Data of drug treatment are revealed as increasing rate (%) against the control.
Target diseases of assay Immune system, Allergy
Assay level Cell-based
Protocol of assay 1. Cultivation of MODE-K cells with IFN-γ 100 unit/ml for 72 hr
2. Cultivation of MODE-K cells with Mitomycin C 50 ug/ml for 45 min
3. Cultivation for 2 hr after onto the 96-well plates
4. Cultivation for 1 hr after addition of test agents
5. Cultivation for 5 hr with Lipofectine and DMEM containing HEL
6. 3A9 cells onto 96-well plates (5×10^4/well)
7. Measurement of IL-2 concentration by ELISA after 24 hr
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
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