Menu  → 【Exploratory Research】Methods of bioassay  → 
Stimulatory and Inhibitory Effects on hydrolysis activity of hyaluronidase
Stimulatory and Inhibitory Effects on hydrolysis activity of hyaluronidase

Exploratory Research

Measured value Ratio to control (%)
Animal species Others
Indication of the result The ratio (%) of hydrolysis activity of hyaluronidase under test drug treatment to that for the control condition without test drug is shown. Values less than 100 indicate inhibition of hydrolysis activity of hyaluronidase.
Target diseases of assay Others
Assay level Cell-free
Protocol of assay 1) 5 μl of test drug, 12.5 μl of buffer, and 27.5 μl hyarulonic acid (HA) solution as a substrate were mixed in a microtube. 2) 10 μl of bovine testicular hyaluronidase (BTH) was added, and the reaction mixture was incubated for 1 h at 37 °C. 50 μl of the reaction mixture contained sodium acetate buffer (final concentration 100 mM, pH 5.0)-150 mM NaCl, HA (Mr=80,000) 10 μg, and hyaluronidase 10 μg. 3) The reaction was stopped by boiling for 5 min. 4) The reaction products were analyzed by HPLC monitored at 215 nm. Hydrolysis activity of hyaluronidase was assessed according to the peak areas of HA tetra- and hexasaccharides (HA4 and HA6, respectively), which were major final products.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

TOPICS

Information on genetic analysis

Metabolism information on chemical compounds included in crude drugs

Traditional Medical & Pharmaceutical Database


Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
2630 Sugitani, Toyama 930-0194 Japan
TEL: +81-76-434-7601
FAX: +81-76-434-5064