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Stimulatory effect on secondary metabolite production by Aspergillus fumigatus
Stimulatory effect on secondary metabolite production by Aspergillus fumigatus

Exploratory Research

Measured value Others
Animal species Others
Indication of the result Changes in HPLC profile of culture extract against the control condition with water: 2 new peak(s) appeared; 1 area(s) of existing peak(s) changed; 0 no change.
Target diseases of assay Others
Assay level Cell-based
Protocol of assay 1) 2 mL of potate dextrose broth was dispensed to 24-well plates and Aspergillus fumigatus was inoculated to the plate. 2) 120 μL of testing crude drug extract was added to each well. The fungi were cultured at 25 °C for 1 week. 3) The plates were freezedried and metabolites were extracted with 1.5 mL of methanol at each well. 4) The extracts were dissolved at 1 mL of 50 % acetonitrile and were analyzed using HPLC.
Remarks  
【Results of bioassay】
Display method Crude drug Kampo formula Compound
Crude drug-Compound
Name of experimental material
(compound/crude drug/Kampo formula)

 

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Division of Pharmacognosy,
Department of Medicinal Resources,
Institute of Natural Medicine,
University of Toyama
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