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Saikosaponin
Saikosaponin

Compound Saikosaponin 
Animal species human intestinal microflora 
Metabolism parameters Oral administration
Oral administration
Oral administration
Oral administration
Oral administration
Oral administration
Oral administration
Oral administration
Oral administration
Metabolites Saikosaponin a
Prosaikogenin F
Saikogenin F
Saikosaponin b
Prosaikogenin A
Saikogenin A
Saikosaponin c
Saikogenin E
Crude drug Bupleurum Root 
References 1) Kida H., Nakamura N., Meselhy M. R., Akao T. and Hattori M.: Isolation and identification of human intestinal bacteria capable of hydrolyzing saikosaponins. J. Trad. Med., 14, 34-40 (1997).

2) Kida H., Akao T., Meselhy M. R. and Hattori M.: Enzymes responsible for the metabolism of saikosaponins from Eubacterium sp. A-44, a human intestinal anaerobe.
Biol. Pharm. Bull., 20, 1274-1278 (1997).

3) Kida H., Akao T., Meselhy M. R. and Hattori M.: Metabolism and pharmacokinetics of orally administered saikosaponin b1 in conventional, germ-free and Eubacterium sp. A-44 infected gnotobiote rats. Biol. Pharm. Bull., 21, 588-593 (1998). 
Remarks ※Preparation of a bacterial suspension of human feces
Fresh feces obtained from a healthy young man (age : 25, male) were suspended in five volumes of phosphate buffer (pH 7.2). The fecal suspension thus obtained was used in the following experiments. [Kida et al., J. Trad. Med., 14, 34-40 (1997)]

※Time course of the metabolism of saikosaponins by an intestinal bacterial suspension : GAM broth (9 ml) containing saikosaponin a (1), b1 (3), b2 (4), c (5) or d (2) (a final concentration, 1 mM) was incubated with an intestinal bacterial suspension (1 ml) in an anaerobic incubator at 37°C. A 100 μm portion was taken out at intervals (4, 10, 24 and 48 hours) and extracted with BuOH (100 μl). Five microliters of the BuOH layer were applied to a TLC plate, which was developed with solvent system A. [Kida et al., J. Trad. Med., 14, 34-40 (1997)] 
 

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