Saikosaponin b2
|
|
| Compound | Saikosaponin b2 |
| Animal species | human intestinal microflora |
| Metabolism parameters | |
| Metabolites |
Saikosaponin b2
Prosaikogenin D Saikogenin D |
| Crude drug | Bupleurum Root |
| References | 1) Kida H., Nakamura N., Meselhy M. R., Akao T. and Hattori M.: Isolation and identification of human intestinal bacteria capable of hydrolyzing saikosaponins. J. Trad. Med., 14, 34-40 (1997). |
| Remarks | ※Preparation of a bacterial suspension of human feces Fresh feces obtained from a healthy young man (age: 25, male) were suspended in five volumes of phosphate buffer (pH 7.2). The fecal suspension thus obtained was used in the following experiments. [Kida et al., J. Trad. Med., 14, 34-40 (1997)] ※Time course of the metabolism of saikosaponin b2 by an intestinal bacterial suspension GAM broth (9 ml) containing saikosaponin b2 (a final concentration, 1 mM) was incubated with an intestinal bacterial suspension (1 ml) in an anaerobic incubator at 37°C. A 100 μm portion was taken out at intervals (4, 10, 24 and 48 hours) and extracted with BuOH (100 μl). Five micro liters of the BuOH layer were applied to a TLC plate, which was developed with solvent system A. [Kida et al., J. Trad. Med., 14, 34-40 (1997)] |
