Benzoylaconine
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| 化合物名 | Benzoylaconine |
| 動物種 | ラット |
| 代謝パラメータ | |
| 代謝物 |
Aconitine
Benzoylaconine |
| 関連生薬 | 附子 |
| 参考文献 | 1) Tazawa T., Zhao H., Li Y., Meselhy M. R., Nakamura N., Akao T. and Hattori M.: A new enzyme immunoassay for aconitine and its application to quantitative determination of aconitine levels in plasma. Biol. Pharm. Bull., 26, 1289-1294 (2003). 2) Zhao Z., Sun X. F., Nakamura N., Zuo F., Yang X. W., and Hattori M.: Development of an enzyme immunoassay system for mesaconitine and its application to the disposition study on mesaconitine. J. Trad. Med., 20, 201-207 (2003). 3) Zuo F., Zhao J., Nakamura N., Gao J. J., Akao T., Hattori M., Oomiga Y., and Kikuchi Y.: Pharmakokinetic stydy of benzoylmesaconine in rats using an enzyme immunoassay system. J. Nat. Med., 60, 313-321 (2006). |
| 論文備考 | ※Disposition of aconitine (1) after oral administration in rats One hour after oral administration of aconitine (1) at doses of 0.8, 0.2, 0.05 mg/kg in rats, under general anesthesia, the blood and spinal cord (L1-L4) were collected, and their aconitine and benzoylaconine contents were quantitatively determined by the respective enzyme immunoassay systems. [Tazawa et al., Biol. Pharm. Bull., 26, 1289-1294 (2003); Zuo et al., J. Nat. Med., 60, 313-321 (2006)] ※Enzyme immunoassay for benzoylaconine (2) The immunization protocol included primary inoculation of three female albino rabbits with DBAE-BSA (2 mg) conjugate in saline (2 ml) emulsified with the same volume of complete Freund's adjuvant. After two weeks, the rabbits were continued with booster injections at two weeks for 3 months and three weeks' interval for two months. The emulsion was prepared with 1.5 mg of the conjugate in saline (1.5 ml) and incomplete Freund's adjuvant (1.5 ml). ※Samples or standard solutions (50 μl) containing various amounts of benzoylmesaconine (2) were incubated with an antiserum (50 μl) and a 103-fold diluted β-Gal conjugate (25 μl) at room temperature for 2 h. Ten-fold diluted goat anti-rabbit IgG (50 μl) and 100-fold diluted normal rabbit serum (20 μl) were then added to the reaction mixture. The mixture was left overnight at 4°C. After addition of buffer A (1 ml), the resulting mixture was centrifuged at 1100 × g for 20 min at 4°C. The supernatant was discarded, and the precipitates were washed with buffer A followed by incubation with 0.1 mM 4-methylumbelliferyl β-D-galactoside for 30 min at 30°C. The reaction was stopped by the addition of 100 mM glycine-NaOH buffer, pH 10.3 (3 ml). The fluorescence intensity of a product (4-methylumbelliferone) was spectrofluorometrically measured at wavelengths of 365 nm (excitation) and 448 nm (emission). Zuo et al., J. Nat. Med., 60, 313-321 (2006)] |
