Rheinoside A, B
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| 化合物名 | Rheinoside A, B |
| 動物種 | ヒト腸内細菌フローラ |
| 代謝パラメータ | |
| 代謝物 |
Rheinoside A
8-Deglucosylrheinoside A |
| 関連生薬 | 大黄 |
| 参考文献 | 1) 牧野圭吾修士論文『ヒト腸内細菌によるanthrone及びoxyanthrone C-配糖体のC-グルコシル結合の開裂について』 (2000) 富山医科薬科大学. |
| 論文備考 | ・Metabolism of rheinosides by human intestinal flora A fecal bacterial suspension (0.5 ml) precultured for 24 h was inoculated into PYF broth (4.5 ml) containing 2 mM rheinoside A or B, and the mixture was anaerobically incubated for 24 h. ・Detection for metabolites of rheinosides A and B A 50 ml portion was taken out, and mixed with 50 ml of MeOH containing 0.1% AcOH, followed by centrifugation at 8800 x g for 1 min to separate a supernatant and precipitates. A portion of the supernatant was applied to a normal phase TLC plate of silica gel and developed with a mixed solvent CHCl3-MeOH-H2O (6 : 4 : 1). The metabolite was quantitatively determined by TLC-densitomstry at a wavelength of 350 nm. |
