Genistein
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| Compound | Genistein |
| Animal species | human intestinal bacterium strain PUE |
| Metabolism parameters | |
| Metabolites |
Genistein
5-Hydroxyequol |
| Crude drug | Pueraria Root , Sophora Japonica Flower , Sophora Subprostrata Root |
| References | |
| Remarks | A bacterial suspension (50 ml) of strain DZE was inoculated to 0.8 l GAM broth containing genistein (50 mg) and incubated at 37 °C in an anaerobic incubator for 120 h. The reaction mixture was then extracted three times with ethyl acetate. The organic layer was evaporated under reduced pressure to give a residue. The residue was applied to a column of silica gel, which was eluted with a solvent system, CHCl3–MeOH (20 : 1), to give 5-hydroxyequol (24 mg, 50% in yield). |
