Rheinoside A, B
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| Compound | Rheinoside A, B |
| Animal species | human intestinal microflora |
| Metabolism parameters | |
| Metabolites |
Rheinoside A
8-Deglucosylrheinoside A |
| Crude drug | Rhubarb |
| References | 1) 牧野圭吾修士論文『ヒト腸内細菌によるanthrone及びoxyanthrone C-配糖体のC-グルコシル結合の開裂について』 (2000) 富山医科薬科大学. |
| Remarks | ・Metabolism of rheinosides by human intestinal flora A fecal bacterial suspension (0.5 ml) precultured for 24 h was inoculated into PYF broth (4.5 ml) containing 2 mM rheinoside A or B, and the mixture was anaerobically incubated for 24 h. ・Detection for metabolites of rheinosides A and B A 50 ml portion was taken out, and mixed with 50 ml of MeOH containing 0.1% AcOH, followed by centrifugation at 8800 x g for 1 min to separate a supernatant and precipitates. A portion of the supernatant was applied to a normal phase TLC plate of silica gel and developed with a mixed solvent CHCl3-MeOH-H2O (6 : 4 : 1). The metabolite was quantitatively determined by TLC-densitomstry at a wavelength of 350 nm. |
