Strychinine N
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| Compound | Strychinine N |
| Animal species | human intestinal bacteria Lactobacillus acidophilus |
| Metabolism parameters | |
| Metabolites |
Strychinine N-oxide
16-Hydroxystrychine Strychnine |
| Crude drug | Nux Vomica |
| References | 1) El-Mekkawy S., Meselhy M. R., Kawata Y., Kadota S., Hattori M., and Namba T.: Metabolism of strychnine N-oxide and brucine N-Oxide by human intestinal bacteria. Planta Med., 59, 347-350 (1993). |
| Remarks | ※Screening of bacterial strains for their ability to metabolize strychnine N-oxide (1) Each precultured bacterial suspension (10 ml) was added to 100 ml of GAM broth (pH 7.3) and the mixture was incubated under anaerobic conditions at 37 °C for 24 h. The culture was centrifuged at 7800 x g for 5 min and the pellets were washed 2 times with saline and with 0.1 M phosphate buffer (pH 7.3). The bacterial cells were suspended in 0.1 M phosphate buffer (10 ml, pH 7.3) and 10 mg of strychnine N-oxide (1) in DMSO (0.1 ml) were added to each bacterial suspension. The mixture was anaerobically incubated for 48 h, and adjusted with 8 % Na2CO3 to pH ca. 9, then extracted with EtOAc (10 ml x 2). The extract was evaporated in vacuo to give a residue. The residue was dissolved in CHCl3-MeOH (1:1 v/v, 400 μl) and the metabolites were quantitatively analyzed by HPLC using Develosil ODS Ph 5 C18 column (Nomura Co. Ltd., Seto, Japan, 250 mm x 4.5 mm i.d.); mobile phase, CH3CN-0.03M Na2HPO4 (55:45); flow rate, l.0 ml/min; injection volume, 1 μl; detection, 254nm. The metabolites were analyzed quantitatively using calibration lines pre pared with authentic samples. [El-Mekkawy et al., Planta Med., 59, 347-350 (1993)] |
